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MAST® ICT – Indirect Carbapenemase Test

Carbapenemases are found in organisms that reside in the bowel and are often associated with high mortality rates. They are highly transmissible in healthcare settings and detection is imperative for infection control purposes. Although there are commercial solutions for the detection of carbapenemases in Enterobacteriaceae, options for Pseudomonas and Acinetobacter spp. remain limited.

MAST® has developed MAST®ICT; a paper test device, based on the principles of the Indirect

Carbapenemase Test (ICT). The ICT method utilises a cell permeabilising agent to release carbapenemase enzyme from a Carbapenemase-Producing-Organism (CPO) to hydrolyse an indicator antibiotic in the test system. This allows a normally carbapenem susceptible reporter organism to grow where antibiotic has been hydrolysed, thus producing a distorted zone of inhibition. If the test organism produces no carbapenemase, the reporter organism will form a regular, circular zone of inhibition around the indicator tip.

Interpretation of results

Tip 1/4 and 2/3 –ESBL & AmpC Inhibitors + Porinating agent

Tip X – Penem

 

                                                      Positive                                                                     Negative

Features and Benefits

Detects the (Big-5-Carbapenemases)

MAST® ICT is a reliable screening tool for OXA,KPC,DNM,IMP and VIM enzymes—also known as the ‘Big-5-Carbapenemases’

Convenient format

Each device provided individually wrapped with its own desiccant, allowing the product to be used according to your personal laboratory demand.

Suitable for use with Acinetobacter, Pseudomonas spp. and Enterobacteriaceae

Simple assay for screening of problematic carbapenemase-producers, that is easy to perform and cost-effective.

Interpretation by simple observation

Distortion of the zone around Tip 3 indicates carbarpenemase-production.  No specialist equipment for interpretation.

SALDA MEMBER

Quality System

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We are here


QC

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Commercial Sets offered by Microbiologics contain the manufacturer recommended quality control strains for bacterial identification systems (e.g., Vitek®) and bacterial detection products (e.g., Readycult®). QC Microorganism Sets are available for many instruments including:
  Vitek® and Vitek® 2
  bulletMicroScan®
  bulletAPI®
  bulletBD BBL™ Crystal™, Phoenix™ and GeneOhm™
 bullet Cepheid® Smart Cycler® and GeneXpert®
 bullet Roche LightCycler®
     And Many More!
Call us now for further information

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 A Robust and Reliable Antimicrobial Cartridge Disc Dispenser 

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 Available in a 6 place format, with a simple single hand dispenser also available for occasional cartridge users.

  bulletSimple to use operation.

    bulletReliable and robust performance to cope with high throughput workload    

Audible location of cartridges in dispenser   

bulletPositive placement of antibiotic susceptibility discs onto agar surface.    

bulletHeight adjustable to suit variable depths of culture media. 

bulletSeal tight container for protection against moisture.

 Call us now for further information.